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beas 2b cell  (ATCC)


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    ATCC beas 2b cell
    Beas 2b Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beas+2b/BEAS-2B/pm42320433-336-0-7
    Average 99 stars, based on 3986 article reviews
    beas 2b cell - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Single-cell and spatial transcriptomic profiling of POU5F1 in Lung Adenocarcinoma: Dynamics, spatial niche, and prognosis via multi-algorithm ML.
    Article Snippet: .. The following cell lines from the American Type Culture Collection (ATCC) were used: BEAS-2B (normal bronchial epithelium), A549 (lung adenocarcinoma), HCC827 (lung adenocarcinoma, EGFR mutant), NCIH1975 (lung adenocarcinoma, EGFR T790M mutant), NCI-H2228 (lung adenocarcinoma, EML4-ALK fusion), and Calu-1 (lung squamous cell carcinoma). .. Cell culture conditions were strictly standardized: A549 and Calu-1 were cultured in DMEM medium (Hyclone), BEAS-2B in Bronchial Epithelial Cell Growth Medium (BEGM, Lonza), and the remaining cells in RPMI-1640 medium (Gibco).

    Cell Culture:

    Article Title: Dioscin alleviates allergic airway inflammation with IL-4R-associated modulation of epithelial-immune responses.
    Article Snippet: Objective: Allergic asthma is characterized by airway inflammation, epithelial barrier dysfunction, and dysregulated immune responses.. While Th2 cytokines and IL-4 receptor (IL-4R) signaling are central to its pathogenesis, accumulating evidence underscores the involvement of epithelial–immune interactions, metabolic disturbances, and gut–lung axis dysregulation.. Dioscin, a natural steroidal saponin, has demonstrated antiinflammatory properties, but its role in airway inflammation remains insufficiently explored.

    Article Title: Network-based single-cell analysis identifies a proliferative GZMB + plasma cell subset linked to Hedgehog signaling and prognostic risk in lung squamous cell carcinoma.
    Article Snippet: .. 166 AR TIC LE IN PR ES S 2.5 Supplementary experimental methods 167 2.5.1 Cell culture 168 The BEAS-2B, A549, HCC827, Calu-1, NCI-H2228 and NCI-H1975 cell lines were procured from 169 the American Cell Bank (ATCC). .. The A549 and Calu-1 cells were cultured in DMEM (Hyclone, 170 USA), the BEAS-2B cells were grown in bronchial epithelial cell growth medium (BEGM, Lonza, 171 USA), and the HCC827, NCI-H2228 and NCI-H1975 cells were cultured in RPMI-1640 medium 172 (Gibco, Thermo Fisher Scientific, USA).

    Article Title: Repurposing drugs for EGFR-targeted cancer therapy: An in silico and in vitro study with pharmacophore-based insights.
    Article Snippet: Cancer is the second leading cause of death globally and remains a priority due to its impact on life quality, treatment complexity, and high costs.. To expedite drug development, researchers are increasingly repurposing FDA-approved drugs and clinical candidates, reducing time and costs through in silico methods.. In this study, 3235 FDA-approved and clinical molecules were screened for EGFR inhibition, a significant target due to its role in cancer progression and treatment resistance.

    Modification:

    Article Title: Repurposing drugs for EGFR-targeted cancer therapy: An in silico and in vitro study with pharmacophore-based insights.
    Article Snippet: Cancer is the second leading cause of death globally and remains a priority due to its impact on life quality, treatment complexity, and high costs.. To expedite drug development, researchers are increasingly repurposing FDA-approved drugs and clinical candidates, reducing time and costs through in silico methods.. In this study, 3235 FDA-approved and clinical molecules were screened for EGFR inhibition, a significant target due to its role in cancer progression and treatment resistance.



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    Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. <t>(A)</t> <t>BEAS-2B</t> human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.
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    ATCC normal human bronchial epithelial cell lines
    Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. <t>(A)</t> <t>BEAS-2B</t> human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.
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    ATCC beas 2b cells
    Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. <t>(A)</t> <t>BEAS-2B</t> human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.
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    Image Search Results


    Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. (A) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.

    Journal: Frontiers in Immunology

    Article Title: Integrative multi-omics reveals that downregulation of HLA-DPA1/DPB1 drives macrophage immune-metabolic dysregulation in pediatric asthma

    doi: 10.3389/fimmu.2026.1835475

    Figure Lengend Snippet: Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. (A) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.

    Article Snippet: BEAS-2B (normal human bronchial epithelial) cells were purchased from ATCC and maintained in DMEM at 37 °C.

    Techniques: Biomarker Discovery, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot